Single-cell analysis of salt-induced hypertensive mouse aortae reveals cellular heterogeneity and state changes.

ABSTRACT: Elevated blood pressure caused by excessive salt intake is common and associated with cardiovascular diseases in most countries. However, the composition and responses of vascular cells in the progression of hypertension have not been systematically described. We performed single-cell RNA sequencing on the aortic arch from C57BL/6J mice fed a chow/high-salt diet. We identified 19 distinct cell populations representing 12 lineages, including smooth muscle cells (SMCs), fibroblasts, endothelial cells (ECs), B cells, and T cells. During the progression of hypertension, the proportion of three SMC subpopulations, two EC subpopulations, and T cells increased. In two EC clusters, the expression of reactive oxygen species-related enzymes, collagen and contractility genes was upregulated. Gene set

SUBMITTER: Zhang K 

PROVIDER: S-EPMC8741768 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature