Automating microsatellite screening and primer design from multi-individual libraries using Micro-Primers.

ABSTRACT: Analysis of intra- and inter-population diversity has become important for defining the genetic status and distribution patterns of a species and a powerful tool for conservation programs, as high levels of inbreeding could lead into whole population extinction in few generations. Microsatellites (SSR) are commonly used in population studies but discovering highly variable regions across species' genomes requires demanding computation and laboratorial optimization. In this work, we combine next generation sequencing (NGS) with automatic computing to develop a genomic-oriented tool for characterizing SSRs at the population level. Herein, we describe a new Python pipeline, named Micro-Primers, designed to identify, and design PCR primers for amplification of SSR loci from a multi-individual

SUBMITTER: Alves F 

PROVIDER: S-EPMC8741888 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature