Epac-2 ameliorates spontaneous colitis in Il-10^-/- mice by protecting the intestinal barrier and suppressing NF-κB/MAPK signalling.

ABSTRACT: Intestinal barrier dysfunction and intestinal inflammation interact in the progression of Crohn's disease (CD). A recent study indicated that Epac-2 protected the intestinal barrier and had anti-inflammatory effects. The present study examined the function of Epac-2 in CD-like colitis. Interleukin-10 gene knockout (Il-10^-/- ) mice exhibit significant spontaneous enteritis and were used as the CD model. These mice were treated with Epac-2 agonists (Me-cAMP) or Epac-2 antagonists (HJC-0350) or were fed normally (control), and colitis and intestinal barrier structure and function were compared. A Caco-2 and RAW 264.7 cell co-culture system were used to analyse the effects of Epac-2 on the cross-talk between intestinal epithelial cells and inflammatory cells. Epac-2 activation signi

SUBMITTER: Song X 

PROVIDER: S-EPMC8742196 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature