Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells.

ABSTRACT:

Background

The cytoskeleton is a key component of the system responsible for transmitting mechanical cues from the cellular environment to the nucleus, where they trigger downstream responses. This communication is particularly relevant in embryonic stem (ES) cells since forces can regulate cell fate and guide developmental processes. However, little is known regarding cytoskeleton organization in ES cells, and thus, relevant aspects of nuclear-cytoskeletal interactions remain elusive.

Results

We explored the three-dimensional distribution of the cytoskeleton in live ES cells and show that these filaments affect the shape of the nucleus. Next, we evaluated if cytoskeletal components indirectly modulate the binding of the pluripotency transcription factor OCT4 to chromatin ta

SUBMITTER: Romero JJ 

PROVIDER: S-EPMC8742348 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature