Combined assembly of long and short sequencing reads improve the efficiency of exploring the soil metagenome.

ABSTRACT:

Background

Advances in DNA sequencing technologies have transformed our capacity to perform life science research, decipher the dynamics of complex soil microbial communities and exploit them for plant disease management. However, soil is a complex conglomerate, which makes functional metagenomics studies very challenging.

Results

Metagenomes were assembled by long-read (PacBio, PB), short-read (Illumina, IL), and mixture of PB and IL (PI) sequencing of soil DNA samples were compared. Ortholog analyses and functional annotation revealed that the PI approach significantly increased the contig length of the metagenomic sequences compared to IL and enlarged the gene pool compared to PB. The PI approach also offered comparable or higher species abundance than either PB or IL alo

SUBMITTER: Xu G 

PROVIDER: S-EPMC8742384 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature