Characterization of Anopheles gambiae D7 salivary proteins as markers of human-mosquito bite contact.

ABSTRACT:

Background

Malaria is transmitted when infected Anopheles mosquitoes take a blood meal. During this process, the mosquitoes inject a cocktail of bioactive proteins that elicit antibody responses in humans and could be used as biomarkers of exposure to mosquito bites. This study evaluated the utility of IgG responses to members of the Anopheles gambiae D7 protein family as serological markers of human-vector contact.

Methods

The D7L2, D7r1, D7r2, D7r3, D7r4 and SG6 salivary proteins from An. gambiae were expressed as recombinant antigens in Escherichia coli. Antibody responses to the salivary proteins were compared in Europeans with no prior exposure to malaria and lifelong residents of Junju in Kenya and Kitgum in Uganda where the intensity of malaria transmission is moderat

SUBMITTER: Oseno B 

PROVIDER: S-EPMC8742437 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature