Investigation of red clover (Trifolium pratense) isoflavonoid residual complexity by off-line CCS-qHNMR.

ABSTRACT: The importance of Trifolium pratense L. as a dietary supplement and its use in traditional medicine prompted the preparation of a thorough metabolite profile. This included the identification and quantitation of principal constituents as well as low abundant metabolites that constitute the residual complexity (RC) of T. pratense bioactives. The purity and RC of isoflavonoid fractions from standardized red clover extract (RCE) was determined using an off-line combination of countercurrent separation (CCS) and two orthogonal analytical methodologies: quantitative ¹H NMR spectroscopy with external calibration (EC-qHNMR) and LC-MS. A single-step hydrostatic CCS methodology (Centrifugal Partition Chromatography [CPC]) was developed that fractionated the isoflavonoids with a hexanes-e

SUBMITTER: Malca-Garcia GR 

PROVIDER: S-EPMC8742771 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature