A new variant of the human α-lactalbumin-oleic acid complex as an anticancer agent for chronic myeloid leukemia.

ABSTRACT: Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of hematopoietic stem cells. Although there have been advancements in treatment, there is still a need to develop a biotherapeutic agent. A new variant of the human alpha-lactalbumin-oleic acid (HALOA) complex has been synthesized, which showed similarities with SNARE. The native α-LA was treated with EDTA to remove Ca²⁺ ions confirmed by ICP-OES and Arsenazo III to unfold and attain apo structure. The apo LA was mixed with OA in a specific ratio, leading to HALOA complex formation. The conformational state from native to complex was elucidated by circular dichroism (far; 190-260 nm and near; 260-340 nm UV-CD), which confirmed that the complex consists of a majority of turns and β-sheet structure. SDS-PAGE re

SUBMITTER: Singh V 

PROVIDER: S-EPMC8742887 | biostudies-literature | 2021 Sep-Oct

REPOSITORIES: biostudies-literature