Rapid Highly-Efficient Digestion and Peptide Mapping of Adeno-Associated Viruses.

ABSTRACT: Adeno-associated viruses (AAVs) comprise an area of rapidly growing interest due to their ability to act as a gene delivery vehicle in novel gene therapy strategies and vaccine development. Peptide mapping is a common technique in the biopharmaceutical industry to confirm the correct sequence, product purity, post-translational modifications (PTMs), and stability. However, conventional peptide mapping is time-consuming and has proven difficult to reproduce with viral capsids because of their high structural stability and the suboptimal localization of trypsin cleavage sites in the AAV protein sequences. In this study, we present an optimized peptide mapping-based workflow that provides thorough characterization within 1 day. This workflow is also highly reproducible due to its simplicity h

SUBMITTER: Toole EN 

PROVIDER: S-EPMC8743033 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature