Integrating in vitro metabolomics with a 96-well high-throughput screening platform.

ABSTRACT:

Introduction

High-throughput screening (HTS) is emerging as an approach to support decision-making in chemical safety assessments. In parallel, in vitro metabolomics is a promising approach that can help accelerate the transition from animal models to high-throughput cell-based models in toxicity testing.

Objective

In this study we establish and evaluate a high-throughput metabolomics workflow that is compatible with a 96-well HTS platform employing 50,000 hepatocytes of HepaRG per well.

Methods

Low biomass cell samples were extracted for metabolomics analyses using a newly established semi-automated protocol, and the intracellular metabolites were analysed using a high-resolution spectral-stitching nanoelectrospray direct infusion mass spectrometry (nESI-DIMS) method

SUBMITTER: Malinowska JM 

PROVIDER: S-EPMC8743266 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature