Whole set of constitutive promoters for RpoN sigma factor and the regulatory role of its enhancer protein NtrC in Escherichia coli K-12.

ABSTRACT: The promoter selectivity of Escherichia coli RNA polymerase (RNAP) is determined by its promoter-recognition sigma subunit. The model prokaryote E. coli K-12 contains seven species of the sigma subunit, each recognizing a specific set of promoters. Using genomic SELEX (gSELEX) screening in vitro, we identified the whole set of 'constitutive' promoters recognized by the reconstituted RNAP holoenzyme alone, containing RpoD (σ⁷⁰), RpoS (σ³⁸), RpoH (σ³²), RpoF (σ²⁸) or RpoE (σ²⁴), in the absence of other supporting regulatory factors. In contrast, RpoN sigma (σ⁵⁴), involved in expression of nitrogen-related genes and also other cellular functions, requires an enhancer (or activator) protein, such as NtrC, fo

SUBMITTER: Shimada T 

PROVIDER: S-EPMC8743547 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature