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Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation.

ABSTRACT: We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.

SUBMITTER: Bosse JT 

PROVIDER: S-EPMC8743550 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Complete genome for <i>Actinobacillus pleuropneumoniae</i> serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation.

Bossé Janine T JT   Li Yanwen Y   Cohen Liza Miriam LM   Stegger Marc M   Angen Øystein Ø   Lacouture Sonia S   Gottschalk Marcelo M   Lei Liancheng L   Koene Miriam M   Kuhnert Peter P   Bandara Aloka B AB   Inzana Thomas J TJ   Holden Matthew T G MTG   Harris David D   Oshota Olusegun O   Maskell Duncan J DJ   Tucker Alexander W AW   Wren Brendan W BW   Rycroft Andrew N AN   Langford Paul R PR   On Behalf Of The BRaDP T Consortium  

Microbial genomics 20211101 11

We report here the complete genome sequence of the widely studied <i>Actinobacillus pleuropneumoniae</i> serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the <i>degS</i  ...[more]

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