Massively parallel transposon mutagenesis identifies temporally essential genes for biofilm formation in Escherichia coli.

ABSTRACT: Biofilms complete a life cycle where cells aggregate, grow and produce a structured community before dispersing to colonize new environments. Progression through this life cycle requires temporally controlled gene expression to maximize fitness at each stage. Previous studies have largely focused on identifying genes essential for the formation of a mature biofilm; here, we present an insight into the genes involved at different stages of biofilm formation. We used TraDIS-Xpress, a massively parallel transposon mutagenesis approach using transposon-located promoters to assay the impact of disruption or altered expression of all genes in the genome on biofilm formation. We identified 48 genes that affected the fitness of cells growing in a biofilm, including genes with known roles an

SUBMITTER: Holden ER 

PROVIDER: S-EPMC8743551 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature