Accuracy of multitarget indirect enzyme-linked immunoassay assay for detection of tuberculosis antibody.

ABSTRACT:

Background

Diagnosis of tuberculosis (TB) is still difficult. The development of rapid and sensitive laboratory tools for the diagnosis of tuberculosis is a priority. This study aimed to develop an indirect enzyme-linked immunoassay (ELISA) assay for detection of TB antibody and explore its diagnostic value in patients with pulmonary tuberculosis (PTB) via a multi-center clinical evaluation.

Methods

The specific antigen, fusion antigen, and specific antibody peptide were obtained using molecular cloning and phage peptide library screening. An indirect ELISA assay was developed using multiple target materials. Further, the assay was validated in six institutions with clinically confirmed TB patients, non-TB patients with pulmonary disease, and healthy controls as research sub

SUBMITTER: Hu Y 

PROVIDER: S-EPMC8743715 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature