Healing of bone defects by induced pluripotent stem cell-derived bone marrow mesenchymal stem cells seeded on hydroxyapatite-zirconia.

ABSTRACT:

Background

Induced pluripotent stem cells (iPSCs) can generate bone marrow mesenchymal stem cells (BMSCs) as seed cells for tissue-engineered bone to repair bone defects. In this study, we investigated the effects of hydroxyapatite-zirconia (HA/ZrO₂) composites combined with iPSC-derived BMSCs as a bone substitute on repairing skull defects in rats.

Methods

Human urinary cells isolated from a healthy donor were reprogramed into iPSCs and then induced into BMSCs. Immunocytochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) were used to examine the characteristics of the induced MSCs. The iPSC-derived BMSCs were cultured on HA/ZrO₂ composites, and cytocompatibility of the composite was analyzed by cell counting kit-8 (CCK-8) assa

SUBMITTER: Zhou L 

PROVIDER: S-EPMC8743724 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature