Expanded validation of the effect and quality of a pathogen inactivation system based on riboflavin photochemistry on platelet bacterial contamination.

ABSTRACT:

Background

Bacterial contamination still poses serious challenges to blood safety. Platelets have the highest bacterial contamination risk of all blood components.

Methods

Twenty units of manual platelets were prepared from blood donated by our hospital, which were inoculated with Staphylococcus aureus and Escherichia coli suspensions. The riboflavin sodium phosphate solution was added into platelets, adjusted to a final concentration of 160 μmol/L. Platelets added into an illumination bag and placed in the inactivation system for riboflavin photochemistry at various doses. The inactivation effect of bacteria was evaluated on a Columbia blood agar plate by the plate counting method. Meanwhile, the blood routine, blood gas analysis, platelet aggregation test, an

SUBMITTER: Fan B 

PROVIDER: S-EPMC8743726 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature