Thrombospondin-1 aggravates colonic mucosal inflammatory injuries via promoting the differentiation of CD11c⁺ macrophages with lysosomal activity limited in colitis.

ABSTRACT:

Background

Increased CD11c⁺ Mφ aggravates colonic mucosal injuries in ulcerative colitis (UC) with TSP1 protein increased. The thrombospondin-1 (TSP1) protein which could activate Mφ is closely related to the colonic mucosal damage in UC. Here, we investigated the role of TSP1 in the differentiation of CD11c⁺ Mφ and the mechanism.

Methods

We analyzed the population characteristics of TSP1 genes using the Genotype-Tissue Expression (GTEx) database, and human serum TSP1 protein was detected with ELISA. DSS-induced colitis rats were used to explore the effects of TSP1 on colonic mucosal inflammation. We analyzed the serum cytokines and tissue histopathology to evaluate the severity of UC. Furthermore, we analysed the main source of TSP1 in colon tis

SUBMITTER: Tao P 

PROVIDER: S-EPMC8743729 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature