Bio-electrospraying assessment toward in situ chondrocyte-laden electrospun scaffold fabrication.

ABSTRACT: Electrospinning has been widely used to fabricate fibrous scaffolds for cartilage tissue engineering, but their small pores severely restrict cell infiltration, resulting in an uneven distribution of cells across the scaffold, particularly in three-dimensional designs. If bio-electrospraying is applied, direct chondrocyte incorporation into the fibers during electrospinning may be a solution. However, before this approach can be effectively employed, it is critical to identify whether chondrocytes are adversely affected. Several electrospraying operating settings were tested to determine their effect on the survival and function of an immortalized human chondrocyte cell line. These chondrocytes survived through an electric field formed by low needle-to-collector distances and low voltage.

SUBMITTER: Semitela A 

PROVIDER: S-EPMC8743920 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature