Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer.

ABSTRACT:

Background

Breast cancer is the most common cancer, and the leading cause of cancer-related deaths, among females world-wide. Recent research suggests that extracellular vesicles (EVs) play a major role in the development of breast cancer metastasis. Axillary lymph node dissection (ALND) is a procedure in patients with known lymph node metastases, and after surgery large amounts of serous fluid are produced from the axilla. The overall aim was to isolate and characterize EVs from axillary serous fluid, and more specifically to determine if potential breast cancer biomarkers could be identified.

Methods

Lymphatic drain fluid was collected from 7 patients with breast cancer the day after ALND. EVs were isolated using size exclusion chromatography, quantified and detected by na

SUBMITTER: Ekstrom K 

PROVIDER: S-EPMC8744234 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature