The interactome of CLUH reveals its association to SPAG5 and its co-translational proximity to mitochondrial proteins.

ABSTRACT:

Background

Mitochondria require thousands of proteins to fulfill their essential function in energy production and other fundamental biological processes. These proteins are mostly encoded by the nuclear genome, translated in the cytoplasm before being imported into the organelle. RNA binding proteins (RBPs) are central players in the regulation of this process by affecting mRNA translation, stability, or localization. CLUH is an RBP recognizing specifically mRNAs coding for mitochondrial proteins, but its precise molecular function and interacting partners remain undiscovered in mammals.

Results

Here we reveal for the first time CLUH interactome in mammalian cells. Using both co-IP and BioID proximity-labeling approaches, we identify novel molecular partners interacting sta

SUBMITTER: Hemono M 

PROVIDER: S-EPMC8744257 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature