Reproducibility and flexibility of monoclonal antibody production with Nicotiana benthamiana.

ABSTRACT: The ongoing SARS-CoV-2 coronavirus pandemic of 2020-2021 underscores the need for manufacturing platforms that can rapidly produce monoclonal antibody (mAb) therapies. As reported here, a platform based on Nicotiana benthamiana produced mAb therapeutics with high batch-to-batch reproducibility and flexibility, enabling production of 19 different mAbs of sufficient purity and safety for clinical application(s). With a single manufacturing run, impurities were effectively removed for a representative mAb product (the ZMapp component c4G7). Our results show for the first time the reproducibility of the platform for production of multiple batches of clinical-grade mAb, manufactured under current Good Manufacturing Practices, from Nicotiana benthamiana. The flexibility of the syst

SUBMITTER: Swope K 

PROVIDER: S-EPMC8744878 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature