Impact of growth media and pressure on the diversity and antimicrobial activity of isolates from two species of hexactinellid sponge.

ABSTRACT: Access to deep-sea sponges brings with it the potential to discover novel antimicrobial candidates, as well as novel cold- and pressure-adapted bacteria with further potential clinical or industrial applications. In this study, we implemented a combination of different growth media, increased pressure and high-throughput techniques to optimize recovery of isolates from two deep-sea hexactinellid sponges, Pheronema carpenteri and Hertwigia sp., in the first culture-based microbial analysis of these two sponges. Using 16S rRNA gene sequencing for isolate identification, we found a similar number of cultivable taxa from each sponge species, as well as improved recovery of morphotypes from P. carpenteri at 22-25 °C compared to other temperatures, which allows a grea

SUBMITTER: Koch MJ 

PROVIDER: S-EPMC8744994 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature