HPLC-MS/MS method applied to an untargeted metabolomics approach for the diagnosis of "olive quick decline syndrome".

ABSTRACT: Olive quick decline syndrome (OQDS) is a disorder associated with bacterial infections caused by Xylella fastidiosa subsp. pauca ST53 in olive trees. Metabolic profile changes occurring in infected olive trees are still poorly investigated, but have the potential to unravel reliable biomarkers to be exploited for early diagnosis of infections. In this study, an untargeted metabolomic method using high-performance liquid chromatography coupled to quadrupole-time-of-flight high-resolution mass spectrometry (HPLC-ESI-Q-TOF-MS) was used to detect differences in samples (leaves) from healthy (Ctrl) and infected (Xf) olive trees. Both unsupervised and supervised data analysis clearly differentiated the groups. Different metabolites have been identified as potential specific biomarkers, and their

SUBMITTER: Di Masi S 

PROVIDER: S-EPMC8748322 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature