Chronic pancreatitis in a caerulein-induced mouse model is associated with an altered gut microbiome.

ABSTRACT:

Background

Chronic pancreatitis (CP) is an inflammatory disease of the pancreas with loss of exocrine/endocrine functions as well as development of fibrosis. Dysbiosis of gut microbiome has been shown to be involved in the pathogenesis of many disease processes. Therefore, we aim to investigate the alteration in gut microbiome associated with CP in caerulein-induced mouse model.

Methods

CP was induced in C57Bl/6 by using caerulein injections (50 μg/kg/h, i.p., x7, twice weekly for 10 weeks). Stool samples were collected either one week after end of injection (10-week CP) or 6 weeks (16-week CP). DNA was extracted from stool samples and V4 region of 16S rDNA was sequenced for microbiome analysis.

Results

CP was strongly associated with the alteration in the composition

SUBMITTER: Tao J 

PROVIDER: S-EPMC8748396 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature