The immune-suppressive landscape in lepromatous leprosy revealed by single-cell RNA sequencing.

ABSTRACT: Lepromatous leprosy (L-LEP), caused by the massive proliferation of Mycobacterium leprae primarily in macrophages, is an ideal disease model for investigating the molecular mechanism of intracellular bacteria evading or modulating host immune response. Here, we performed single-cell RNA sequencing of both skin biopsies and peripheral blood mononuclear cells (PBMCs) of L-LEP patients and healthy controls. In L-LEP lesions, we revealed remarkable upregulation of APOE expression that showed a negative correlation with the major histocompatibility complex II gene HLA-DQB2 and MIF, which encodes a pro-inflammatory and anti-microbial cytokine, in the subset of macrophages exhibiting a high expression level of LIPA. The exhaustion of CD8⁺ T cells featured by the high expression of TIGI

SUBMITTER: Mi Z 

PROVIDER: S-EPMC8748782 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature