Ontology highlight
ABSTRACT:
SUBMITTER: Dayalan Naidu S
PROVIDER: S-EPMC8749459 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Dayalan Naidu Sharadha S Suzuki Takafumi T Dikovskaya Dina D Knatko Elena V EV Higgins Maureen M Sato Miu M Novak Miroslav M Villegas José A JA Moore Terry W TW Yamamoto Masayuki M Dinkova-Kostova Albena T AT
iScience 20211227 1
Transcription factor Nrf2 and its negative regulator Keap1 orchestrate a cytoprotective response against oxidative, metabolic, and inflammatory stress. Keap1 is a drug target, with several small molecules in drug development. Here, we show that the isoquinoline PRL-295 increased Keap1 thermostability in lysates from cells expressing fluorescently tagged Keap1. The thermostability of endogenous Keap1 also increased in intact cells and murine liver following PRL-295 treatment. Fluorescence Lifetim ...[more]