2-Trifluoromethyl-6-mercurianiline Nucleotide, a Sensitive ¹⁹F NMR Probe for Hg(II)-mediated Base Pairing.

ABSTRACT: A 2-trifluoromethylaniline C-nucleoside was synthesized, incorporated in the middle of an oligonucleotide, and mercurated. The affinity of the mercurated oligonucleotide toward complementary strands placing each of the canonical nucleobases opposite to the organomercury nucleobase analogue was examined by ultraviolet (UV), circular dichroism (CD), and ¹⁹F NMR spectroscopy analyses. According to the UV melting profile analysis, the organomercury nucleobase analogue showed increased affinities in the order T > G > C > A. The CD profiles indicated the typical B-type helix in each case. The ¹⁹F resonance signal proved sensitive for the local environmental changes, showing clearly distinct signals for the duplexes with different opposing nucleobases. Furthermore, valuable

SUBMITTER: Aro-Heinila A 

PROVIDER: S-EPMC8749955 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature