Active Site Hydrogen Bonding Induced in Cytochrome P450cam by Effector Putidaredoxin.

ABSTRACT: Cytochrome P450s are diverse and powerful catalysts that can activate molecular oxygen to oxidize a wide variety of substrates. Catalysis relies on effective uptake of two electrons and two protons. For cytochrome P450cam, an archetypal member of the superfamily, the second electron must be supplied by the redox partner putidaredoxin (Pdx). Pdx also plays an effector role beyond electron transfer, but after decades the mechanism remains under investigation. We applied infrared spectroscopy to heme-ligated CN^- to examine the influence of Pdx binding. The results indicate that Pdx induces the population of a conformation wherein the CN^- ligand forms a strong hydrogen bond to a solvent water molecule, experimentally corroborating the formation of a proposed proton delive

SUBMITTER: Mammoser CC 

PROVIDER: S-EPMC8750573 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature