DNA Polymerase η Promotes the Transcriptional Bypass of N²-Alkyl-2'-deoxyguanosine Adducts in Human Cells.

ABSTRACT: To cope with unrepaired DNA lesions, cells are equipped with DNA damage tolerance mechanisms, including translesion synthesis (TLS). While TLS polymerases are well documented in facilitating replication across damaged DNA templates, it remains unknown whether TLS polymerases participate in transcriptional bypass of DNA lesions in cells. Herein, we employed the competitive transcription and adduct bypass assay to examine the efficiencies and fidelities of transcription across N²-alkyl-2'-deoxyguanosine (N²-alkyl-dG, alkyl = methyl, ethyl, n-propyl, or n-butyl) lesions in HEK293T cells. We found that N²-alkyl-dG lesions strongly blocked transcription and elicited CC → AA tandem mutations in nascent transcripts, where adenosi

SUBMITTER: Tan Y 

PROVIDER: S-EPMC8751221 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature