Targeted Quantitative Profiling of GTP-Binding Proteins Associated with Metastasis of Melanoma Cells.

ABSTRACT: Metastasis is a major obstacle in the therapeutic intervention of melanoma, and several GTP-binding proteins were found to play important roles in regulating cancer metastasis. To assess systematically the regulatory roles of these proteins in melanoma metastasis, we employed a targeted chemoproteomic method, which relies on the application of stable isotope-labeled desthiobiotin-GTP acyl phosphate probes in conjunction with scheduled multiple-reaction monitoring (MRM), for profiling quantitatively the GTP-binding proteins. Following probe labeling, tryptic digestion, and affinity pull-down of desthiobiotin-conjugated peptides, differences in expression levels of GTP-binding proteins in two matched pairs of primary/metastatic melanoma cell lines were measured using liquid chromatography-MR

SUBMITTER: Cai R 

PROVIDER: S-EPMC8751237 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature