Genome-Scale Metabolic Modelling of Lifestyle Changes in Rhizobium leguminosarum.

ABSTRACT: Biological nitrogen fixation in rhizobium-legume symbioses is of major importance for sustainable agricultural practices. To establish a mutualistic relationship with their plant host, rhizobia transition from free-living bacteria in soil to growth down infection threads inside plant roots and finally differentiate into nitrogen-fixing bacteroids. We reconstructed a genome-scale metabolic model for Rhizobium leguminosarum and integrated the model with transcriptome, proteome, metabolome, and gene essentiality data to investigate nutrient uptake and metabolic fluxes characteristic of these different lifestyles. Synthesis of leucine, polyphosphate, and AICAR is predicted to be important in the rhizosphere, while myo-inositol catabolism is active in undifferentiated nodule bacteria in

SUBMITTER: Schulte CCM 

PROVIDER: S-EPMC8751395 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature