Identification and Quantification of Glutathionylated Cysteines under Ischemic Stress.

ABSTRACT: Ischemia reperfusion injury contributes to adverse cardiovascular diseases in part by producing a burst of reactive oxygen species that induce oxidations of many muscular proteins. Glutathionylation is one of the major protein cysteine oxidations that often serve as molecular mechanisms behind the pathophysiology associated with ischemic stress. Despite the biological significance of glutathionylation in ischemia reperfusion, identification of specific glutathionylated cysteines under ischemic stress has been limited. In this report, we have analyzed glutathionylation under oxygen-glucose deprivation (OGD) or repletion of nutrients after OGD (OGD/R) by using a clickable glutathione approach that specifically detects glutathionylated proteins. Our data find that palmitate availability induc

SUBMITTER: Yapa Abeywardana M 

PROVIDER: S-EPMC8751622 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature