Identification and characterisation of SARS-CoV-2 and Human alphaherpesvirus 1 from a productive coinfection in a fatal COVID-19 case.

ABSTRACT:

Background

During routine Coronavirus disease 2019 (COVID-19) diagnosis, an unusually high viral load was detected by reverse transcription real-time polymerase chain reaction (RT-qPCR) in a nasopharyngeal swab sample collected from a patient with respiratory and neurological symptoms who rapidly succumbed to the disease. Therefore we sought to characterise the infection.

Objectives

We aimed to determine and characterise the etiological agent responsible for the poor outcome.

Methods

Classical virological methods, such as plaque assay and plaque reduction neutralisation test combined with amplicon-based sequencing, as well as a viral metagenomic approach, were performed to characterise the etiological agents of the infection.

Findings

Plaque assay revealed two

SUBMITTER: Herlinger AL 

PROVIDER: S-EPMC8752051 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature