Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis.

ABSTRACT:

Background

Leishmaniasis is a neglected tropical disease caused by the parasite Leishmania braziliensis, commonly found in Brazil and associated with cutaneous and visceral forms of this disease. Like other organisms, L. braziliensis has an enzyme called glutamine synthetase (LbGS) that acts on the synthesis of glutamine from glutamate. This enzyme plays an essential role in the metabolism of these parasites and can be a potential therapeutic target for treating this disease.

Objectives

Investigate LbGS structure and generate structural models of the protein.

Methods

We use the method of crosslinking mass spectrometry (XLMS) and generate structural models in silico using I-TASSER.

Findings

42 XLs peptides were identified, of which 37 are explained in a monomeri

SUBMITTER: de Lima JY 

PROVIDER: S-EPMC8752055 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature