Emergence of Clinical Clostridioides difficile Isolates With Decreased Susceptibility to Vancomycin.

ABSTRACT:

Background

Clostridioides difficile infection (CDI) is a leading cause of hospital-associated antibiotic-related diarrhea and deaths worldwide. Vancomycin is one of the few antibiotics recommended for both nonsevere and severe CDI cases. We sought to determine whether vancomycin nonsusceptible C. difficile strains are circulating in the patient population.

Methods

Stool samples from patients with CDI were collected from 438 and 98 patients at a large university hospital in Houston, Texas, and Nairobi, Kenya, respectively. The stools were examined for the presence of vancomycin and metronidazole nonsusceptible C. difficile using broth dilution culture, Etest (BioMérieux, France), polymerase chain reaction (PCR), whole-genome sequencing, and in vivo testing in a CDI mouse mode

SUBMITTER: Darkoh C 

PROVIDER: S-EPMC8752249 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature