Cytosolic phosphofructokinases are important for sugar homeostasis in leaves of Arabidopsis thaliana.

ABSTRACT:

Background and aims

ATP-dependent phosphofructokinases (PFKs) catalyse phosphorylation of the carbon-1 position of fructose-6-phosphate, to form fructose-1,6-bisphosphate. In the cytosol, this is considered a key step in channelling carbon into glycolysis. Arabidopsis thaliana has seven genes encoding PFK isoforms, two chloroplastic and five cytosolic. This study focuses on the four major cytosolic isoforms of PFK in vegetative tissues of A. thaliana.

Methods

We isolated homozygous knockout individual mutants (pfk1, pfk3, pfk6 and pfk7) and two double mutants (pfk1/7 and pfk3/6), and characterized their growth and metabolic phenotypes.

Key results

In contrast to single mutants and the double mutant pfk3/6 for the hypoxia-responsive isoforms, the double mutant pfk1/7 h

SUBMITTER: Perby LK 

PROVIDER: S-EPMC8752397 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature