[Combined detection of Helicobacter pylori 16S rRNA and cagA gene in saliva specimens using multiplex PCR].

ABSTRACT:

Objective

To establish a multiplex PCR-based method for detecting Helicobacter pylori (Hp) 16S rRNA gene and cagA gene in saliva samples for investigating the prevalence of Hp in the oral cavity of Hp-infected patients with digestive tract diseases.

Methods

Bioinformatics technique was used to design specific primers for Hp 16S rRNA and cagA genes for Hp detection using multiplex PCR, with recombinant cloning plasmids serving as the standard positive control. Oral saliva samples were collected from 156 patients with digestive tract diseases, and Hp 16S rRNA and cagA genes were detected using the established multiplex PCR system.

Results

The established multiplex PCR system showed a strong specificity and a high sensitivity for detecting Hp

SUBMITTER: Wang X 

PROVIDER: S-EPMC8752427 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature