[Isolation, identification and safety evaluation of Lactobacillus gasseri strain LGV03 isolated from the vagina of healthy women].

ABSTRACT:

Objective

To characterize the bacterial strain LGV03 isolated from the reproductive tract of healthy women and evaluate its safety.

Methods

The bacterial strains were isolated and purified using MRS agar by dilution plating and streak plate method. Gram staining was used to observe the morphology of the isolated strains and identify Gram-negative and -positive strains. The physiological and biochemical properties of the bacteria was assessed using VITEK ANC card. Strain LGV03 was identified by molecular biology and phylogenetic analysis and tested for hemolysis on blood agar plates. E-test method was performed to examine the sensitivity of strain LGV03. The concentration of lactic acid in the culture supernatant of LGV03 was measured using a biochemical analyzer. The safety

SUBMITTER: Su B 

PROVIDER: S-EPMC8752435 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature