Multisite phosphorylation by Cdk1 initiates delayed negative feedback to control mitotic transcription.

ABSTRACT: Cell-cycle progression is driven by the phosphorylation of cyclin-dependent kinase (Cdk) substrates.^1-3 The order of substrate phosphorylation depends in part on the general rise in Cdk activity during the cell cycle,^4-7 together with variations in substrate docking to sites on associated cyclin and Cks subunits.^3,6,8-10 Many substrates are modified at multiple sites to provide more complex regulation.^10-14 Here, we describe an elegant regulatory circuit based on multisite phosphorylation of Ndd1, a transcriptional co-activator of budding yeast genes required for mitotic progression.^11,12 As cells enter mitosis, Ndd1 phosphorylation by Cdk1 is known to promote mitotic cy

SUBMITTER: Asfaha JB 

PROVIDER: S-EPMC8752490 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature