High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr^-/-.Leiden mouse model.

ABSTRACT: Obesity can disturb spermatogenesis and subsequently affect male fertility and reproduction. In our study, we aim to elucidate at which cellular level of adult spermatogenesis the detrimental effects of obesity manifest. We induced high fat diet (HFD) obesity in low-density lipoprotein receptor knock-out Leiden (Ldlr^-/-.Leiden) mice, and studied the morphological structure of the testes and histologically examined the proportion of Sertoli cells, spermatocytes and spermatids in the seminiferous tubules. We examined sperm DNA damage and chromatin condensation and measured plasma levels of leptin, testosterone, cholesterol and triglycerides. HFD-induced obesity caused high plasma leptin and abnormal testosterone levels and induced an aberrant intra-tubular organisation (ITO) which

SUBMITTER: Komninos D 

PROVIDER: S-EPMC8752771 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature