Viscoelastic parameterization of human skin cells characterize material behavior at multiple timescales.

ABSTRACT: Countless biophysical studies have sought distinct markers in the cellular mechanical response that could be linked to morphogenesis, homeostasis, and disease. Here, an iterative-fitting methodology visualizes the time-dependent viscoelastic behavior of human skin cells under physiologically relevant conditions. Past investigations often involved parameterizing elastic relationships and assuming purely Hertzian contact mechanics, which fails to properly account for the rich temporal information available. We demonstrate the performance superiority of the proposed iterative viscoelastic characterization method over standard open-search approaches. Our viscoelastic measurements revealed that 2D adherent metastatic melanoma cells exhibit reduced elasticity compared to their normal counterpart

SUBMITTER: Parvini CH 

PROVIDER: S-EPMC8752830 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature