Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies.

ABSTRACT: Influenza A viruses cause seasonal epidemics and global pandemics, representing a considerable burden to healthcare systems. Central to the replication cycle of influenza viruses is the viral RNA-dependent RNA polymerase which transcribes and replicates the viral RNA genome. The polymerase undergoes conformational rearrangements and interacts with viral and host proteins to perform these functions. Here we determine the structure of the 1918 influenza virus polymerase in transcriptase and replicase conformations using cryo-electron microscopy (cryo-EM). We then structurally and functionally characterise the binding of single-domain nanobodies to the polymerase of the 1918 pandemic influenza virus. Combining these functional and structural data we identify five sites on the polymerase which

SUBMITTER: Keown JR 

PROVIDER: S-EPMC8752864 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature