Neonatal Maternal Separation Alters Gelatinase Activity in Mouse Ovarian Preantral Follicles.

ABSTRACT:

Objective

The early life environment is critical for normal growth and development for future reproductive function. This study aims to investigate the effect of neonatal maternal separation (MS) on gelatinase activity of mouse ovarian follicles.

Materials and methods

In this experimental study, infants from female NMRI mice were randomly allocated into two groups immediately after birth: i. MS isolated from their mothers for 6 hours per day, from postpartum days 2 to 16) and ii. Control (undisturbed during the 16 days). Ovarian tissues were dissected to perform differential counts of the ovarian follicle type by haematoxylin and eosin staining. The isolated follicles were cultured for 12 days. Gelatinase activity and the gene expressions of matrix metalloproteinases, MMP

SUBMITTER: Asghari Z 

PROVIDER: S-EPMC8753102 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature