Objective Quantitation of EGFR Protein Levels using Quantitative Dot Blot Method for the Prognosis of Gastric Cancer Patients.

ABSTRACT:

Purpose

An underlying factor for the failure of several clinical trials of anti-epidermal growth factor receptor (EGFR) therapies is the lack of an effective method to identify patients who overexpress EGFR protein. The quantitative dot blot method (QDB) was used to measure EGFR protein levels objectively, absolutely, and quantitatively. Its feasibility was evaluated for the prognosis of overall survival (OS) of patients with gastric cancer.

Materials and methods

Slices of 2×5 μm from formalin-fixed paraffin-embedded gastric cancer specimens were used to extract total tissue lysates for QDB measurement. Absolutely quantitated EGFR protein levels were used for the Kaplan-Meier OS analysis.

Results

EGFR protein levels ranged from 0 to 772.6 pmol/g (n=246) for all gastri

SUBMITTER: Xin L 

PROVIDER: S-EPMC8753283 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature