Quantitative Proteomic Analysis Revealed Broad Roles of N⁶-Methyladenosine in Heat Shock Response.

ABSTRACT: As optimum temperature is essential for all living organisms, heat shock represents a challenging problem for their survival. Therefore, cellular response to heat shock is among the most extensively investigated stress response pathways; however, how the human proteome responds to heat shock has not been comprehensively investigated. In this study, we employed stable isotope labeling by amino acids in cell culture (SILAC), together with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, to fulfill an in-depth analysis of the alterations in the human proteome in M14 human melanoma cells in response to heat shock stress. We found that, after heat shock, 284 and 278 out of the 4319 quantified proteins were with substantially diminished and elevated expressions, respectively.

SUBMITTER: Miao W 

PROVIDER: S-EPMC8753891 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature