Presence of Malassezia Hyphae Is Correlated with Pathogenesis of Seborrheic Dermatitis.

ABSTRACT: Seborrheic dermatitis (SD) is a common, chronic, and relapsing skin disease. The roles of Malassezia spp. in the pathogenesis of SD are still not clear due to the lack of direct evidence for the existence of hyphae within affected skin tissues. We set out to elucidate if Malassezia mycelium contributes to the onset and development of SD and if Malassezia mycelium is correlated with the clinical severity of SD patients. We detected Malassezia hyphae in patients with SD using potassium hydroxide (KOH) and calcofluor white (CFW) staining. Fluorescent microscopy was performed for the analysis of fungal cell wall and morphological characteristics of Malassezia under CFW staining. Culture growth in modified Dixon agar was used for DNA extraction and sequencing,

SUBMITTER: Li J 

PROVIDER: S-EPMC8754105 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature