Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity.

ABSTRACT: Preclinical studies of primary cancer cells are typically done after tumors are removed from patients or animals at ambient atmospheric oxygen (O₂, ~21%). However, O₂ concentrations in organs are in the ~3 to 10% range, with most tumors in a hypoxic or 1 to 2% O₂ environment in vivo. Although effects of O₂ tension on tumor cell characteristics in vitro have been studied, these studies are done only after tumors are first collected and processed in ambient air. Similarly, sensitivity of primary cancer cells to anticancer agents is routinely examined at ambient O₂. Here, we demonstrate that tumors collected, processed, and propagated at physiologic O₂ compared to ambient air display distinct differences in key signaling networ

SUBMITTER: Kumar B 

PROVIDER: S-EPMC8754301 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature