Clinical Utilization of DiaSorin Molecular Polymerase Chain Reaction in Pneumocystis Pneumonia.

ABSTRACT:

Background

Pneumocystis jirovecii polymerase chain reaction (PCR) testing is a sensitive diagnostic tool but does not distinguish infection from colonization. Cycle threshold (C_T) may correlate with fungal burden and could be considered in clinical decision making. Clinical use of PCR and significance of C_T values have not previously been examined with the DiaSorin Molecular platform.

Methods

Retrospective review of P jirovecii PCR, C_T values and clinical data from 18 months in a multihospital academic health system. The diagnostic performance of PCR with respect to pathology and correlation of C_T with severity were examined.

Results

Ninety-nine of 1006 (9.8%) assays from 786 patients in 919 encounters were pos

SUBMITTER: Damhorst GL 

PROVIDER: S-EPMC8754379 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature