CUT&Tag-BS for simultaneous profiling of histone modification and DNA methylation with high efficiency and low cost.

ABSTRACT: It remains a challenge to decipher the complex relationship between DNA methylation, histone modification, and the underlying DNA sequence with limited input material. Here, we developed an efficient, low-input, and low-cost method for the simultaneous profiling of genomic localization of histone modification and methylation status of the underlying DNA at single-base resolution from the same cells in a single experiment by integrating cleavage under targets and tagmentation (CUT&Tag) with tagmentation-based bisulfite sequencing (CUT&Tag-BS). We demonstrated the validity of our method using representative histone modifications of euchromatin and constitutive and facultative heterochromatin (H3K4me1, H3K9me3, and H3K27me3, respectively). Similar histone modification enrichment patterns were

SUBMITTER: Li R 

PROVIDER: S-EPMC8754398 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature