Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos.

ABSTRACT: Regulatory interactions between enhancers and core promoters are fundamental for the temporal and spatial specificity of gene expression in development. The central role of core promoters is to initiate productive transcription in response to enhancer's activation cues. However, it has not been systematically assessed how individual core promoter elements affect the induction of transcriptional bursting by enhancers. Here, we provide evidence that each core promoter element differentially modulates functional parameters of transcriptional bursting in developing Drosophila embryos. Quantitative live imaging analysis revealed that the timing and the continuity of burst induction are common regulatory steps on which core promoter elements impact. We further show that the upstream TATA also af

SUBMITTER: Yokoshi M 

PROVIDER: S-EPMC8754644 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature